Serological studies of influenza infection among population in southern region of Kazakhstan during the 2018-2021 epidemic season Authors Mira Galautdinovna Shamenova The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan Tatyana Ivanovna Glebova The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0002-1139-9868 Nailya Galiveyevna Klivleyeva The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0002-4094-137X Sagadat Bakhytkyzy Baiseiit The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0002-9305-7254 Asem Maratovna Baimukhametova The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0002-8959-126X Nurbol Temirbayevich Saktaganov The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0001-6526-956X Nuray Syrlybayevna Ongarbayeva The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0001-9022-331X Dariya Azamatovna Ismagulova The Research and Production Center for Microbiology and Virology, Almaty, Kazakhstan https://orcid.org/0000-0002-8959-126X DOI: https://doi.org/10.47391/JPMA.6348 Keywords: Virus, , Influenza, , Antigen, , Serum, , Diagnosis. Abstract Objective: To conduct serological studies of influenza infection rate during an epidemic. Methods: The retrospective study was conducted at the Research and Production Centre for Microbiology and Virology, Almaty, Kazakhstan, and comprised data, including blood samples, from patients with symptoms of acute respiratory viral infection, bronchitis and pneumonia during 2018-21 from various healthcare institutions in the Almaty region. Serological tests on blood serums were carried out sing haem agglutination inhibition assay and enzyme-linked immunosorbent assay. Data was analysed using Graph Pad Prism 9. Results: Of the 779 blood samples, 392(50.3%) came from women and 387(49.7%) from men. The overall age range was 0-80 years. Serological analyses using haem agglutination inhibition assay showed the presence of anti-hemagglutinins against pandemic A(H1N1)pdm09 virus in 292(37.5%) samples, influenza A/H3N2 virus in 340(43.6%) and type B virus in 53(6.8%). Antibodies against two subtypes of influenza A virus and type B virus were simultaneously identified in 25(3.2%) cases, whereas against influenza A (H1N1+H3N2) viruses in 69(8.9%). In enzyme-linked immunosorbent assay, antibodies against influenza A/H1N1pdm virus were detected in 108(13.9%) cases, against A/H3N2 virus in 105(13.5%) and type B virus in 65(8.3%). Antibodies simultaneously against two subtypes of influenza A virus were identified in 46(5.9%) of blood serums, and against influenza A and B viruses in 60(7.7%). Conclusion: Co-circulation of influenza A and B viruses was observed, confirming the role of influenza viruses in the epidemic process. Key Words: Virus, Influenza, Antigen, Serum, Diagnosis. Downloads Full Text Article Published 2023-03-15 How to Cite Shamenova, M. G., Glebova, T. I., Klivleyeva, N. G., Baiseiit, S. B., Baimukhametova, A. M., Saktaganov, N. T., Ongarbayeva, N. S., & Ismagulova , D. A. (2023). Serological studies of influenza infection among population in southern region of Kazakhstan during the 2018-2021 epidemic season. Journal of the Pakistan Medical Association, 73(4), 804–807. https://doi.org/10.47391/JPMA.6348 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 73 No. 4 (2023): April Section RESEARCH ARTICLE License Copyright (c) 2023 Journal of the Pakistan Medical Association This work is licensed under a Creative Commons Attribution 4.0 International License.