Oxidative stress and PON1 (T172A/L55M) polymorphism: potential risk factors for Osteoarthritis Authors Tuba Abid Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi, Pakistan Syeda Abiha Zehra Jaffari Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi, Pakistan Muhammad Zohaib Department of Molecular Pathology, Dow University of Health Sciences, Karachi, Pakistan Muhammad Jamil Department of Orthopaedic Surgery, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan Sadia Afzal Department of Biochemistry, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan Zehra Hashim Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi, Pakistan DOI: https://doi.org/10.47391/JPMA.3658 Abstract Objective: To investigate the role of oxidative stress in the progression of osteoarthritis with the genetic determinant of paraoxonase-1 enzyme L55M in osteoarthritis patients. Method: The case control study was carried out at the University of Karachi from April to November 2020, and comprised blood samples of female osteoarthritis patients aged >50 years and healthy controls matched for age and gender. Oxidative stress was assessed by measuring lipid peroxidation product and protein carbonyl content. Activities of paraoxonase-1 paraoxonase and arylesterase were evaluated in the subjects. Protein expression of paraoxonase-1 was also analysed using western blot method. Paraoxonase-1 L55M (rs854560) polymorphism was determined using tetra-amplification-refractory mutation system polymerase chain reaction. Data was analysed using SPSS® Statistics 20.0. Results: Of the 103 subjects, 50(48.5%) were patients and 53(51.5%) were controls. The overall age range was 50-70 years. The extent of malondialdehyde (p<0.001) and protein carbonyl content (p<0.05) were increased significantly in the patients compared to the controls. Activity of paraoxonase and arylesterase was found decreased (p<0.001) in patients compared to the controls. The prevalence of genotype MM was higher in the patients than the controls(p=0.001). L55M was more pronounced in patients suffering than the controls (p=0.01). Conclusion: The elevated levels of malondialdehyde and cabonylated protein content might be associated with osteoarthritis progression. Decreased serum paraoxonase-1 activity with L55M was the major consequence of oxidative stress in female osteoarthritis patients. Key Words: Osteoarthritis, Oxidative stress, Paraoxonase, Polymorphism. Author Biography Muhammad Zohaib, Department of Molecular Pathology, Dow University of Health Sciences, Karachi, Pakistan Current Affiliation: Department of Molecular Pathology, Dow University of Health Sciences, Karachi. Downloads Full Text Article Published 2022-09-15 How to Cite Abid, T., Jaffari, S. A. Z., Muhammad Zohaib, Muhammad Jamil, Afzal, S. ., & Hashim, Z. (2022). Oxidative stress and PON1 (T172A/L55M) polymorphism: potential risk factors for Osteoarthritis. Journal of the Pakistan Medical Association, 72(10), 1971–1976. https://doi.org/10.47391/JPMA.3658 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 72 No. 10 (2022): OCTOBER Section RESEARCH ARTICLE